THE HERO........

THE HERO........

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Senin, Februari 6

Materi BIOKIMIA D3 ANKES UNIMUS


Hari / Tanggal             : Kamis, 1 Oktober 2015
Materi                          : Test Molish,Fehling dan Benedict
Waktu                         : 13.40 – 15.20 Wib
Tujuan                         : 1. Test Molish : Menguji adanya karbohidrat                                                                         2. Test Fehling   : Mengetahui gula pereduksi dan non pereduksi                                                3. Test Benedict : Mengetahui gula pereduksi dan non pereduksi    
Prinsip
·         Test Molish                                                                                                                                        Asam sulfat pekat menghidrolisa ikatan glikosida merubah monosakarida menjadi furfural dan derivate-derivate nya,kemudian akan bergabung  dengan  a, naptol  tersulfonasi  menghasilkan kompleks berwarna purple ( ungu)
·         Test Fehling
Reaksi reduksi gula
·         Test Benedict
Reaksi reduksi gula ( modifikasi test Fehling )
Alat
·         Tabung Reaksi
·         Rak Tabung Reaksi
·         Pipet Tetes
·         Pembakar spirtus
·         Penjepit Tabung
·         Penagas air
·         Gelas Ukur 10 ml
Bahan
·         Larutan a-napthol   1%
·         Larutan H2SO4
·         Larutan Fehling A
·         Larutan Fehling B
·         Larutan benedict
·         Larutan amylum     1%
·         Larutan glukosa      1%
·         Larutan fruktosa     1%
·         Larutan maltosa      1%
·         Larutan laktosa       1%
·         Larutan sukrosa      1%
·         Larutan galaktosa   1%


       Hari/Tanggal               : kamis,8 oktober 2015                                                                                                           Materi                          : Barfoed,Seliwanof, Test iodin                                                                                                                                                   Waktu                         : 13,40 – 15.20 wib                                                                                                                                                Tujuan                         : 1. Test barfoed          : Untuk membedakan monosakarida dan disakarida
                                       2. Test Seliwanof     : Untuk mengetahui adanya gugus altosa dan ketosa
                                       3. Test Iodin             : Untuk mengetahui sifat dari polisakarida
Prinsip                         :
Test Barfoed               : Reagen barfoed merupakan asam lemak dan hanya direduksi oleh monosakarida.Pemanasan yang lama akan menghidrolisa disakarida reaksi positif  palsu
Test Seliwanof            : Reaksi asam pekat( Hcl ) dengan larutan gula dapat membentuk 4 hidroksi-metal                                                                                                    furfural dan dengan resolsinol ( 1,3-dihidroksi benzena membentuk warna merah)
Test Iodin                    : Iodium membentuk warna kompleks dengan polisakarida tepung memberikan warna Biru pada Iodium,glikogen dan tepung yang sudah di hidrolisis sebagian (entrodekstrin) memberikan warna merah sampe coklat dengan Iodium
Alat
1.Tabung reaksi                                                                                                                                                   2. Rak tabung reaksi                                                                                                                                            3. Pipet tetes                                                                                                                                                                  4. Penjepit tabung                                                                                                                                                      5. Pemanas air                                                                                                                                                     7. Plat tetes
       Bahan
1. Lar barfoed                                                                                                                                                      2. Lar glukosa                                                                                                                                                                3. Lar fruktosa                                                                                                                                                               4. Lar galaktosa                                                                                                                                                 5. Lar maltosa                                                                                                                                                                 6. Lar sukrosa                                                                                                                                                                 7. Lar seliwanof                                                                                                                                                     8. Lar Iodin                                                                                                                                                                  9. Lar Hcl                                                                                                                                                      10. Lar amylum                                                                                                                                               11. Lar Inulin                                                                                                                                                              12. Lar selulosa                                                                                                                                            13. Lar laktosa

Hari/Tanggal               : kamis,15 oktober 2015                                                                                                                 Materi                          : Fermentasi,Karbohidrat, Hidrolisis sukrosa                                                                            Waktu                         : 13,40 – 15.20 wib                                                                                                                                                Tujuan                         :Fermentasi karbohidrat : untuk mengetahuikarbohidrat yang dapat di fermentasi dan                                   yang tidak dapat di fermentasi                                                                                                                    : Hidrolisis sukrosa          : untuk mengetahui hasil hidrolisis sukrosa
                                   
Prinsip                         :                                                                                                                                     Test fermentasi karbohidrat Ragi akan memfermentasi beberapa karbohidrat menghasilkan alkohol dan CO2bila test dilakukan di dalam tabung fermentasi .CO2dapat mudah di deteksi
Alat
1.Tabung fermentasi                                                                                                                                           2. Rak tabung reaksi                                                                                                                                            3. Inkubator                                                                                                                                                                                 4. Tabung reaksi                                                                                                                                           5. Pemanas air                                                                                                                                                                       6. Buffer fosfat
Bahan
1. Ragi roti ( 20% dalam buffer fosfat )                                                                                                                                       2. Glukosa 1%                                                                                                                                                                        3. Fruktosa 1%                                                                                                                                                                   4. galaktosa 1%                                                                                                                                                                     5. Maltosa 1%                                                                                                                                                      6. sukrosa 1%                                                                                                                                                          7. Lar hcl pekat                                                                                                                                                  8. Lar 5 N NaOH                                                                                                                                                                                9. Reagen benedict                                                                                                                                                                        10. Lar Ba (OH)2                                                                                                                                               11. Reagen Barfoed                                                                                                                                                 12. Lar selulosa                                                                                                                                                   13. Lar laktosa




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